MGMT antibody (AA 2-207)

Catalog No. ABIN3042363

Product Details anti-MGMT Antibody

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Binding Specificity: AA 2-207

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB, IHC-P, ICC in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: O-6-methylguanine-DNA methyltransferase
Protein Name: Methylated-DNA--protein-cysteine methyltransferase

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MGMT recombinant protein (Position: D2-N207). Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MGMT is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT (MGMT Products)

Synonyms: AGT antibody, AI267024 antibody, Agat antibody, O-6-methylguanine-DNA methyltransferase antibody, MGMT antibody, Mgmt antibody

Background: O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in humans is encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene. MGMT is mapped to 10q26.3. This gene is crucial for genome stability. It repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. It has been found that methylation of the MGMT promoter in the tumor was associated with longer survival.

Synonyms: 6 O methylguanine DNA methyltransferase antibody|6-O-methylguanine-DNA methyltransferase antibody|Agat antibody|AGT antibody|AI267024 antibody|Methylated DNA protein cysteine methyltransferase antibody|Methylated-DNA--protein-cysteine methyltransferase antibody|Methylguanine DNA methyltransferase antibody|MGC107020 antibody|MGMT antibody|MGMT_HUMAN antibody|O 6 methylguanine DNA alkyltransferase antibody|O 6 methylguanine DNA methyltransferase antibody|O-6-methylguanine-DNA methyltransferase antibody|O-6-methylguanine-DNA-alkyltransferase antibody

Gene ID: 4255

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus