RIPK1 antibody (AA 316-671)

Catalog No. ABIN3042480

Product Details anti-RIPK1 Antibody

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Binding Specificity: AA 316-671

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 1(RIPK1) detection. Tested with WB in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 1(RIPK1) detection. Tested with WB in Human.
Gene Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
Protein Name: Receptor-interacting serine/threonine-protein kinase 1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RIP recombinant protein (Position: K316-N671). Human RIP shares 65% amino acid (aa) sequence identity with mouse RIP.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for RIP is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-RIPK1 antibody (ABIN3042480)

Wang, Ma, Hu, Xie, Wu, Zeng, Song: "Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors." in: BMC cancer, Vol. 16, pp. 545, (2017) (PubMed).

Chen, Zhao, Wu, Zou, Luo, Li, Xie, Liang: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).

Target Details for RIPK1

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Alternative Name: RIPK1 (RIPK1 Products)

Synonyms: RIP antibody, RIP1 antibody, D330015H01Rik antibody, Rinp antibody, Rip1 antibody, rip1 antibody, ripk1 antibody, xRIP1beta antibody, xrip1 antibody, receptor interacting serine/threonine kinase 1 antibody, receptor (TNFRSF)-interacting serine-threonine kinase 1 antibody, receptor interacting serine/threonine kinase 1 L homeolog antibody, RIPK1 antibody, Ripk1 antibody, ripk1.L antibody

Background: RIPK1, also known as RIP or RIP1, is an enzyme that in humans is encoded by the RIPK1 gene. It is mapped to 6p25.2. RIPK1 is a key signaling molecule in the programmed necrosis pathway, which plays important roles in development, tissue damage response, and antiviral immunity. RIPK1 is known to have function in a variety of cellular pathways including the NF-κB pathway and programmed necrotic cell death (necroptosis). The kinase domain, while important for necroptotic (programmed necrotic) functions, it appears dispensable for other lethal, as well as pro-survival roles. Also, proteolytic processing of RIPk1, through both caspase-dependent and -independent mechanisms, triggers lethality that is dependent on the generation of one or more specific C-terminal cleavage product(s) of RIPk1 upon stress.

Synonyms: Cell death protein RIP antibody|FLJ39204 antibody|OTTHUMP00000039163 antibody|Receptor (TNFRSF) interacting serine threonine kinase 1 antibody|receptor interacting protein 1 antibody|Receptor interacting protein antibody|Receptor interacting serine threonine protein kinase 1 antibody|Receptor TNFRSF interacting serine threonine kinase 1 antibody|Receptor-interacting protein 1 antibody|Receptor-interacting serine/threonine-protein kinase 1 antibody|Rinp antibody|RIP 1 antibody|RIP antibody|Rip-1 antibody|RIP1 antibody|RIPK 1 antibody|Ripk1 antibody|RIPK1_HUMAN antibody|Serine threonine protein kinase RIP antibody|Serine/threonine-protein kinase RIP antibody

Gene ID: 8737

UniProt: Q13546

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway