SIRT3 antibody (AA 66-399)

Catalog No. ABIN3042484

Product Details anti-SIRT3 Antibody

Target: SIRT3 (Sirtuin 3 (SIRT3))

Binding Specificity: AA 66-399

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-3, mitochondrial(SIRT3) detection. Tested with WB in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-3, mitochondrial(SIRT3) detection. Tested with WB in Human,Rat.
Gene Name: sirtuin 3
Protein Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for SIRT3 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3 (SIRT3 Products)

Synonyms: zgc:158774 antibody, sirtuin antibody, SIR2L3 antibody, 2310003L23Rik antibody, AI848213 antibody, Sir2l3 antibody, sirtuin 3 antibody, sirt3 antibody, SIRT3 antibody, Sirt3 antibody

Background: NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. It is mapped to 11p15.5. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of SIRT3 in cultured cells increases respiration and decreases the production of reactive oxygen species. SIRT3 can function as a mitochondrial deacetylase. In addition to it, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

Synonyms: hSIRT 3 antibody|hSIRT3 antibody|Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody|NAD dependent deacetylase sirtuin 3 mitochondrial antibody|NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody|Regulatory protein SIR2 homolog 3 antibody|Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody|Sir 2 like 3 antibody|SIR 2 like protein 3 antibody|SIR 3 antibody|SIR2 L3 antibody|Sir2 like 3 antibody|SIR2 like protein 3 antibody|SIR2-like protein 3 antibody|SIR2L3 antibody|SIR3_HUMAN antibody|SIRT 3 antibody|SIRT3 antibody|Sirtuin 3 antibody|Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody|Sirtuin type 3 antibody|Sirtuin3 antibody

Gene ID: 23410