RIPK1 antibody (Middle Region)

Catalog No. ABIN3042930

Cited in 2+ publications. The Rabbit Polyclonal anti-RIPK1 antibody (ABIN3042930) specifically detects RIPK1 in WB. The antibody is reactive with Human samples.

Quick Overview for RIPK1 antibody (Middle Region) (ABIN3042930)

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RIPK1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-RIP/RIPK1 Antibody

Sequence: RRRRVSHDPF AQQRP

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RIP/RIPK1 Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human RIP.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-RIPK1 antibody (ABIN3042930)

Wang, Ma, Hu, Xie, Wu, Zeng, Song: "Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors." in: BMC cancer, Vol. 16, pp. 545, (2017) (PubMed).

Chen, Zhao, Wu, Zou, Luo, Li, Xie, Liang: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).

Target Details for RIPK1

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Alternative Name: RIPK1

Background:

Background: RIPK1 (Regulator of G Protein Signaling 3), also called RIP, is an enzyme that in humans is encoded by the RIPK1 gene. Members of the TRAF protein family have been implicated in the activation of NF-kappa-B by the TNF superfamily. By yeast 2-hybrid and coimmunoprecipitation studies using mammalian cell extracts, Hsu et al. (1996) showed that RIP interacts with TRADD, TRAF1, TRAF2, and TRAF3. Hartz (2012) mapped the RIPK1 gene to chromosome 6p25.2 based on an alignment of the RIPK1 sequence with the genomic sequence. Stanger et al. (1995) found that overexpression of Rip in mammalian cells induced morphologic changes characteristic of apoptosis. They suggested that RIP may be an important element in the signal transduction machinery that mediates programmed cell death.

Gene Full Name: receptor interacting serine/threonine kinase 1

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Molecular Weight: 76 kDa

Gene ID: 8737

UniProt: Q13546

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway