MAP2K6 antibody (N-Term)

Catalog No. ABIN3042996

Product Details anti-MAP2K6 Antibody

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Binding Specificity: AA 7-23, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K6 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 6(MAP2K6) detection. Tested with WB in Human,Mouse,Rat.

Sequence: KKRNPGLKIP KEAFEQP

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 6(MAP2K6) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase 6
Protein Name: Dual specificity mitogen-activated protein kinase kinase 6

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MEK6(7-23aa KKRNPGLKIPKEAFEQP), identical to the related mouse sequence and different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for MAP2K6 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAP2K6

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Alternative Name: MAP2K6 (MAP2K6 Products)

Synonyms: MAPKK6 antibody, MEK6 antibody, MKK6 antibody, PRKMK6 antibody, SAPKK-3 antibody, SAPKK3 antibody, Mkk6 antibody, Prkmk6 antibody, MKK3 antibody, map2k3 antibody, zMKK3 antibody, map2k6 antibody, mitogen-activated protein kinase kinase 6 antibody, mitogen-activated protein kinase kinase 6 L homeolog antibody, MAP2K6 antibody, Map2k6 antibody, map2k6 antibody, map2k6.L antibody

Background: MAP2K6(Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6(MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene, on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein(MAP) kinase kinase. Han et al.(1996) described experiments addressing the function of MKK6 in intracellular signaling pathways leading toward activation of p38 MAPK. Mukherjee et al.(2006) found that the Yersinia YopJ virulence factor acted as an acetyltransferase, using coenzyme A to modify critical ser and thr residues in the MAP2K6 activation loop, thereby blocking phosphorylation and kinase activation. They proposed that ser and/or thr acetylation by a bacterial effector may be a mechanism that occurs in other signaling pathways.

Synonyms: Dual specificity mitogen activated protein kinase kinase 6 antibody|Dual specificity mitogen-activated protein kinase kinase 6 antibody|EC 2.7.12.2 antibody|MAP kinase kinase 6 antibody|MAP2K6 antibody|MAPK/ERK kinase 6 antibody|MAPKK 6 antibody|MAPKK6 antibody|MEK 6 antibody|MEK6 antibody|Mitogen Activated Protein Kinase Kinase 6 antibody|MKK 6 antibody|MKK6 antibody|MP2K6_HUMAN antibody|PRKMK6 antibody|Protein kinase mitogen activated kinase 6 antibody|protein kinase, mitogen-activated, kinase 6(MAP kinase kinase 6) antibody|SAPK kinase 3 antibody|SAPKK-3 antibody|SAPKK3 antibody|SKK3 antibody|Stress-activated protein kinase kinase 3 antibody

UniProt: P52564

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades