RAC1 antibody (C-Term)

Catalog No. ABIN3043366

Product Details anti-RAC1 Antibody

Target: RAC1

Binding Specificity: AA 169-189, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Ras-related C3 botulinum toxin substrate 1(RAC1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: FDEAIRAVLC PPPVKKRKRK C

Cross-Reactivity (Details): Cross-reactivity with Rac2 has not been validated. No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Ras-related C3 botulinum toxin substrate 1(RAC1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Protein Name: Ras-related C3 botulinum toxin substrate 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rac1 (169-189aa FDEAIRAVLCPPPVKKRKRKC), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Rac1 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-RAC1 antibody (ABIN3043366)

Han, Zhuang, Chen, Zhu, Cai, Lu et al.: "Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal dendritic injury in Alzheimer's disease concurrent with cofilin1/phosphorylated-cofilin1 and ..." in: PLoS ONE, Vol. 12, Issue 9, pp. e0185102, (2017) (PubMed).

Shen, Yang, Yao, Sun, Chen, Mo, Shi, Chen, Liu: "Gadolinium promoted proliferation in mouse embryo fibroblast NIH3T3 cells through Rac and PI3K/Akt signaling pathways." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 27, Issue 4, pp. 753-62, (2015) (PubMed).

Target Details for RAC1

Target: RAC1

Abstract: RAC1 Products

Synonyms: Rac-1 antibody, TC-25 antibody, p21-Rac1 antibody, AL023026 antibody, D5Ertd559e antibody, RAC2 antibody, CRAC1A antibody, rac1 antibody, wu:fd16e02 antibody, zgc:55823 antibody, zgc:55917 antibody, zgc:86934 antibody, 2248 antibody, CG2248 antibody, D-Rac antibody, D-Rac 1 antibody, D-Rac1 antibody, DRac antibody, DRac1 antibody, DRacA antibody, Dm Rac1 antibody, DmRAC antibody, DmRAC1 antibody, Dmel\CG2248 antibody, Dmrac1 antibody, Drac antibody, Drac1 antibody, Drac1a antibody, DracA antibody, GTPase Rac1 antibody, P40792 antibody, RAC1 antibody, Rac antibody, Rac GTPase antibody, Rac3 antibody, RacA antibody, dRac antibody, dRac1 antibody, drac1 antibody, rac antibody, Xrac antibody, mig5 antibody, p21-rac1 antibody, tc-25 antibody, Rac family small GTPase 1 antibody, RAS-related C3 botulinum substrate 1 antibody, ras-related C3 botulinum toxin substrate 1 antibody, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody, Rac family small GTPase 1a antibody, CG2248 gene product from transcript CG2248-RB antibody, Rac family small GTPase 1 L homeolog antibody, ras homolog family member G antibody, RAC1 antibody, Rac1 antibody, rac1a antibody, rac1.L antibody, RHOG antibody

Background: Rac1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1gene. This gene can produce a variety of alternatively splicedversions of the Rac1 protein, which appear to carry out different functions. This gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms: Cell migration inducing gene 5 protein antibody|Cell migration-inducing gene 5 protein antibody|MGC111543 antibody|MIG5 antibody|Migration inducing gene 5 antibody|Migration inducing protein 5 antibody|p21 Rac1 antibody|p21-Rac1 antibody|Rac 1 antibody|RAC1 antibody|RAC1_HUMAN antibody|Ras like protein TC25 antibody|Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody|Ras-like protein TC25 antibody|Ras-related C3 botulinum toxin substrate 1 antibody|Rho family small GTP binding protein Rac1 antibody|TC 25 antibody|TC25 antibody

Gene ID: 5879

UniProt: P63000

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling