Angiotensin II Type-1 Receptor antibody (N-Term)

Catalog No. ABIN3043531

Product Details anti-Angiotensin II Type-1 Receptor Antibody

Target: Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))

Binding Specificity: AA 1-28, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiotensin II Type-1 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Type-1 angiotensin II receptor(AGTR1) detection. Tested with WB, IHC-P in Human,Mouse.

Sequence: MILNSSTEDG IKRIQDDCPK AGRHNYIF

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Type-1 angiotensin II receptor(AGTR1) detection. Tested with WB, IHC-P in Human,Mouse.
Gene Name: angiotensin II receptor, type 1
Protein Name: Type-1 angiotensin II receptor

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AGTR1 (1-28aa MILNSSTEDGIKRIQDDCPKAGRHNYIF), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Angiotensin II Type-1 Receptor antibody (ABIN3043531)

Wu, Dai, Ren, Liu, Li, Chang et al.: "Angiotensin II induces apoptosis of human pulmonary microvascular endothelial cells in acute aortic dissection complicated with lung injury patients through modulating the expression of monocyte ..." in: American journal of translational research, Vol. 8, Issue 1, pp. 28-36, (2016) (PubMed).

Liu, Wang, Wu, Zhang: "Effects of angiotensin II receptor antagonist, Losartan on the apoptosis, proliferation and migration of the human pancreatic stellate cells." in: World journal of gastroenterology, Vol. 11, Issue 41, pp. 6489-94, (2006) (PubMed).

Target Details for Angiotensin II Type-1 Receptor

Target: Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))

Alternative Name: AGTR1 (AGTR1 Products)

Synonyms: AG2S antibody, AGTR1A antibody, AGTR1B antibody, AT1 antibody, AT1AR antibody, AT1B antibody, AT1BR antibody, AT1R antibody, AT2R1 antibody, AT2R1A antibody, AT2R1B antibody, HAT1R antibody, 1810074K20Rik antibody, AI551199 antibody, AT1a antibody, Agtr-1a antibody, Agtr1 antibody, Angtr-1a antibody, AT1A antibody, XAT-1 antibody, agtr1-A antibody, agtr1.2 antibody, AT1-R antibody, at1 antibody, AGTR1 antibody, Agtr-1b antibody, Angtr-1b antibody, agtr1 antibody, agtr1-a antibody, agtr1-b antibody, xAT antibody, angiotensin II receptor type 1 antibody, angiotensin II receptor, type 1a antibody, angiotensin II receptor type 1 S homeolog antibody, uncharacterized AGTR1 antibody, angiotensin II receptor, type 1b antibody, angiotensin II receptor type 1 L homeolog antibody, AGTR1 antibody, Agtr1a antibody, agtr1.S antibody, Agtr1 antibody, Agtr1b antibody, agtr1.L antibody

Background: AGTR1 is known as Angiotensin II Type 1 Receptor. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. And it acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. Moreover, this gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed, however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene.

Synonyms: AG2S antibody|Agtr 1 antibody|Agtr1 antibody|AGTR1_HUMAN antibody|Agtr1a antibody|AGTR1B antibody|Ang II antibody| Angiotensin II receptor type 1 antibody|Angiotensin II type-1 receptor antibody|Angiotensin receptor 1 antibody|Angiotensin receptor 1B antibody|AT 1B antibody|AT 1r antibody|AT1 antibody|At1a antibody|AT1AR antibody|AT1B antibody|AT1BR antibody|AT1R antibody|AT2R1 antibody|AT2R1A antibody|AT2R1B antibody|HAT1R antibody|Type 1 angiotensin II receptor antibody|Type 1B angiotensin II receptor antibody|Type-1 angiotensin II receptor antibody

Gene ID: 185

UniProt: P30556

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour