RAD51 antibody (AA 1-258)
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- Target See all RAD51 Antibodies
- RAD51 (DNA Repair Protein Homolog 1 (RAD51))
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Binding Specificity
- AA 1-258
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RAD51 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for DNA repair protein RAD51 homolog 1(RAD51) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for DNA repair protein RAD51 homolog 1(RAD51) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: RAD51 recombinase
Protein Name: DNA repair protein RAD51 homolog 1 - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Rad51 recombinant protein (Position: M1-E258). Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51.
- Isotype
- IgG
- Top Product
- Discover our top product RAD51 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Rad51 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- RAD51 (DNA Repair Protein Homolog 1 (RAD51))
- Alternative Name
- RAD51 (RAD51 Products)
- Synonyms
- BRCC5 antibody, HRAD51 antibody, HsRad51 antibody, HsT16930 antibody, MRMV2 antibody, RAD51A antibody, RECA antibody, CRAD51 antibody, wu:fb38e12 antibody, zgc:77754 antibody, RGD1563603 antibody, ATRAD51 antibody, F22D1.20 antibody, F22D1_20 antibody, RAS associated with diabetes protein 51 antibody, AV304093 antibody, Rad51a antibody, Reca antibody, brcc5 antibody, rad51 antibody, rad51a antibody, reca antibody, xrad51 antibody, RAD51 recombinase antibody, RAS associated with diabetes protein 51 antibody, RAD51 recombinase L homeolog antibody, RAD51 antibody, rad51 antibody, Rad51 antibody, rad51.L antibody
- Background
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DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene. The Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.
Synonyms: BRCA1/BRCA2 containing complex, subunit 5 antibody|BRCC 5 antibody|BRCC5 antibody|DNA repair protein RAD51 homolog 1 antibody|DNA repair protein rhp51 antibody|E coli RecA homolog antibody|Homolog of E coli RecA antibody|homolog of S cerevisiae RAD51 antibody|HRAD51 antibody|HsRad51 antibody|HsT16930 antibody|MRMV2 antibody|Rad 51 antibody|RAD51 antibody|RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody|RAD51 homolog A antibody|RAD51 homolog antibody|RAD51 homolog S. cerevisiae antibody|RAD51 recombinase antibody|RAD51 S cerevisiae homolog antibody|RAD51, S. cerevisiae, homolog of antibody|RAD51_HUMAN antibody|RAD51A antibody|RECA antibody|RecA homolog E. coli antibody|RecA like protein antibody|RecA, E. coli, homolog of antibody|recombination protein A antibody - Gene ID
- 5888
- UniProt
- Q06609
- Pathways
- DNA Damage Repair
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