HRAS antibody (C-Term)

Catalog No. ABIN3043845

This anti-HRAS antibody is a Rabbit Polyclonal antibody detecting HRAS in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for HRAS antibody (C-Term) (ABIN3043845)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HRAS Antibody

Binding Specificity: AA 101-137, C-Term

Purpose: Anti-GTPase HRAS Antibody Picoband®

Sequence: KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLAR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GTPase HRAS Antibody (ABIN3043845). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GTPase HRAS, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
1. "Entrez Gene: v-Ha-ras Harvey rat sarcoma viral oncogene homolog". 2. Russell MW, Munroe DJ, Bric E, Housman DE, Dietz-Band J, Riethman HC, Collins FS, Brody LC (July 1996). "A 500-kb physical map and contig from the Harvey ras-1 gene to the 11p telomere". Genomics 35 (2): 353-60. 3. Wong-Staal F, Dalla-Favera R, Franchini G, Gelmann EP, Gallo RC (July 1981). "Three distinct genes in human DNA related to the transforming genes of mammalian sarcoma retroviruses". Science 213 (4504): 226-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS

Background:

Synonyms: GTPase HRas,H-Ras-1,Ha-Ras,Transforming protein p21,c-H-ras,p21ras,GTPase HRas, N-terminally processed,HRAS,HRAS1,

Tissue Specificity: Widely expressed. .

Background: GTPase HRas, also known as transforming protein p21, is an enzyme that in humans is encoded by the HRAS gene. This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Molecular Weight: 21 kDa

Gene ID: 3265

UniProt: P01112

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling