IGF2R antibody (C-Term)

Catalog No. ABIN3044000

Product Details anti-IGF2R Antibody

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Binding Specificity: AA 2475-2491, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF2R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Cation-independent mannose-6-phosphate receptor(IGF2R) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: TKLVSFHDDS DEDLLHI

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Cation-independent mannose-6-phosphate receptor(IGF2R) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: insulin-like growth factor 2 receptor
Protein Name: Cation-independent mannose-6-phosphate receptor

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Mannose 6 Phosphate Receptor(Cation independent)(2475-2491aa TKLVSFHDDSDEDLLHI), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-IGF2R antibody (ABIN3044000)

Liang, Hao, Zhang, Wu, Wang: "Insulin-like growth factors in endometrioid adenocarcinoma: correlation with clinico-pathological features and estrogen receptor expression." in: BMC cancer, Vol. 12, pp. 262, (2013) (PubMed).

Target Details for IGF2R

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Alternative Name: IGF2R (IGF2R Products)

Synonyms: CD222 antibody, CIMPR antibody, M6P-R antibody, MPR1 antibody, MPRI antibody, AI661837 antibody, CI-MPR antibody, M6P/IGF2R antibody, Mpr300 antibody, mpri antibody, cd222 antibody, cimpr antibody, m6p-r antibody, insulin like growth factor 2 receptor antibody, insulin-like growth factor 2 receptor antibody, IGF2R antibody, Igf2r antibody, igf2r antibody

Background: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.

Synonyms: 300 kDa mannose 6 phosphate receptor antibody|300 kDa mannose 6-phosphate receptor antibody|Cation independent mannose 6 phosphate receptor antibody|Cation-independent mannose-6-phosphate receptor antibody|CD222 antibody|CD222 antigen antibody|CI Man 6 P receptor antibody|CI Man-6-P receptor antibody|CI MPR antibody|CI-MPR antibody|CIMPR antibody|IGF 2 receptor antibody|IGF 2R antibody|IGF II receptor antibody|IGF-II receptor antibody|IGF2 receptor antibody|IGF2R antibody|Insulin like growth factor 2 receptor antibody|Insulin like growth factor II receptor antibody|Insulin-like growth factor 2 receptor antibody|Insulin-like growth factor II receptor antibody|M6P R antibody|M6P/IGF2 receptor antibody|M6P/IGF2R antibody|M6PR antibody|MPR 300 antibody|MPR300 antibody|MPRI antibody|MPRI_HUMAN antibody

UniProt: P11717