CD25 antibody (N-Term)
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- Target See all CD25 (IL2RA) Antibodies
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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Binding Specificity
- AA 39-57, N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CD25 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Interleukin-2 receptor subunit alpha(IL2RA) detection. Tested with WB, IHC-P in Human.
- Sequence
- MAYKEGTMLN CECKRGFRR
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Interleukin-2 receptor subunit alpha(IL2RA) detection. Tested with WB, IHC-P in Human.
Gene Name: interleukin 2 receptor, alpha
Protein Name: Interleukin-2 receptor subunit alpha(IL-2 receptor subunit alpha/IL-2-RA/IL-2R subunit alpha/IL2-RA) - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human CD25/IL-2sR Alpha(39-57aa MAYKEGTMLNCECKRGFRR).
- Isotype
- IgG
- Top Product
- Discover our top product IL2RA Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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- Target
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Alternative Name
- IL2RA (IL2RA Products)
- Background
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IL2RA(Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene.The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped "sushi-like" domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs.Homodimeric alpha chains(IL2RA) result in low-affinity receptor, while homodimeric beta(IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.
Synonyms: CD25 antibody|IDDM10 antibody|IL 2 RA antibody|IL 2 receptor subunit alpha antibodyIL 2R alpha chain antibody|IL 2R subunit alpha antibody|IL-2 receptor subunit alpha antibody|IL-2-RA antibody|IL-2R subunit alpha antibody|IL2 RA antibody|IL2 Receptor alpha antibody|IL2 receptor subunit alpha antibody|IL2-RA antibody|IL2R alpha chain antibody|IL2R antibody|IL2R subunit alpha antibody|IL2RA antibody|IL2RA_HUMAN antibody|Interleukin 2 receptor alpha chain antibody|interleukin 2 receptor subunit alpha antibody|Interleukin 2 receptor, alpha antibody|Interleukin-2 receptor subunit alpha antibody|Ly 43 antibody|Ly43 antibody|OTTHUMP00000019030 antibody|OTTHUMP00000019031 antibody|OTTHUMP00000019032 antibody|p55 antibody|p55 chain antibody|T Cell Growth Factor Receptor antibody|TAC antigen antibody|TCGFR antibody - UniProt
- P01589
- Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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