Integrin beta 3 antibody (C-Term)

Catalog No. ABIN3044227

Product Details anti-Integrin beta 3 Antibody

Target: Integrin beta 3 (ITGB3)

Binding Specificity: AA 752-770, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Integrin beta-3(ITGB3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: EFAKFEEERA RAKWDTANN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Integrin beta-3(ITGB3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: integrin, beta 3(platelet glycoprotein IIIa, antigen CD61)
Protein Name: Integrin beta-3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin beta 3(752-770aa EFAKFEEERARAKWDTANN), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Integrin beta 3 antibody (ABIN3044227)

Qin, Yan, Zhang, Wang, Shen, Lv, Li, Wei, Chen: "TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis." in: Scientific reports, Vol. 6, pp. 20587, (2016) (PubMed).

Wang, Liu, Lin, Wang, Li, Liu, Yan, Zhang, Iwamori: "Study on the expression and clinical significances of lewis y antigen and integrin αv, β3 in epithelial ovarian tumors." in: International journal of molecular sciences, Vol. 12, Issue 6, pp. 3409-21, (2015) (PubMed).

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: ITGB3 (ITGB3 Products)

Synonyms: BDPLT16 antibody, BDPLT2 antibody, CD61 antibody, GP3A antibody, GPIIIa antibody, GT antibody, INGRB3 antibody, integrin antibody, INTB3 antibody, GPIIIA antibody, cd61 antibody, gp3a antibody, itgb3 antibody, itgb3.2 antibody, itgb3.1 antibody, integrin subunit beta 3 antibody, integrin beta 3 antibody, integrin subunit beta 3 L homeolog antibody, integrin beta 3a antibody, integrin beta 3b antibody, ITGB3 antibody, Itgb3 antibody, itgb3.L antibody, itgb3a antibody, itgb3b antibody

Background: ITGB3(INTEGRIN, BETA-3), also called GP3A, GPIIIa, CD61, is a protein that in humans is encoded by the ITGB3 gene. GP3A is a cluster of differentiation found on thrombocytes. The ITGB3 complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. The GP3A gene is mapped to 17q21.32. And the GP3A gene has 14 exons. The 3-prime exon is larger than 1,700 nucleotides and contains the 3-prime untranslated region. The ITGB3 complex mediates platelet aggregation by acting as a receptor for fibrinogen. Although the ITGB3 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the "inside-out" signaling pathways.

Synonyms: BDPLT2 antibody|CD 61 antibody|CD61 antibody|CD61 antigen antibody|GP3A antibody|GPIIIa antibody|GT antibody|HPA 1 antibody|HPA 4 antibody|Integrin beta 3(platelet glycoprotein IIIa antigen CD61) antibody|Integrin beta chain beta 3 antibody|Integrin beta-3 antibody|ITB3_HUMAN antibody|ITG B3 antibody|ITGB 3 antibody|ITGB3 antibody|NAIT antibody|Platelet fibrinogen receptor beta subunit antibody|Platelet glycoprotein IIIa antibody|platelet glycoprotein IIIa precursor antibody|Platelet membrane glycoprotein IIIa antibody|PTP antibody

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex