PDIA3 antibody (Middle Region)

Catalog No. ABIN3044259

Product Details anti-PDIA3 Antibody

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Binding Specificity: AA 172-188, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDIA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Protein disulfide-isomerase A3(PDIA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: LKAASNLRDN YRFAHTN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Protein disulfide-isomerase A3(PDIA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein disulfide isomerase family A, member 3
Protein Name: Protein disulfide-isomerase A3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ERp57(172-188aa LKAASNLRDNYRFAHTN), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-PDIA3 antibody (ABIN3044259)

Shen, Chen, Yang, Chen, Liu, Ni: "Redox proteomics identification of specifically carbonylated proteins in the hippocampi of triple transgenic Alzheimer's disease mice at its earliest pathological stage." in: Journal of proteomics, Vol. 123, pp. 101-13, (2015) (PubMed).

Target Details for PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: PDIA3 (PDIA3 Products)

Synonyms: ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PI-PLC antibody, grp-58 antibody, 58kDa antibody, Erp antibody, Grp58 antibody, PDI antibody, PDI-Q2 antibody, PLC[a] antibody, Plca antibody, 1 antibody, 25D3-membrane-associated antibody, sb:cb825 antibody, erp57 antibody, grp58 antibody, pdia3 antibody, protein disulfide isomerase family A member 3 antibody, protein disulfide-isomerase A3 antibody, protein disulfide isomerase associated 3 antibody, protein disulfide isomerase family A, member 3 antibody, protein disulfide isomerase family A member 3 S homeolog antibody, PDIA3 antibody, Tsp_10062 antibody, Pdia3 antibody, pdia3 antibody, pdia3.S antibody

Background: PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase, however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Synonyms: 58 kDa glucose regulated protein antibody|58 kDa glucose-regulated protein antibody|58 kDa microsomal protein antibody|Disulfide isomerase ER 60 antibody|Disulfide isomerase ER-60 antibody|Endoplasmic reticulum resident protein 57 antibody|Endoplasmic reticulum resident protein 60 antibody|ER p57 antibody|ER protein 57 antibody|ER protein 60 antibody|ERp 57 antibody|ERp57 antibody|ERp60 antibody|ERp61 antibody|Glucose Regulated Protein 58 Kd antibody|GRP 57 antibody|GRP 58 antibody|GRP57 antibody|GRP58 antibody|HsT17083 antibody|P58 antibody|PDIA 3 antibody|PDIA3 antibody|PDIA3_HUMAN antibody|Phospholipase C alpha antibody|PI PLC antibody|Protein disulfide isomerase A3 antibody|Protein disulfide isomerase family A member 3 antibody|Protein disulfide-isomerase A3 antibody

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis