Elastin antibody

Catalog No. ABIN305722

Product Details anti-Elastin Antibody

Target: Elastin (ELN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Brand: IHC-plus™

Specificity: Recognizes human Elastin. Crossreactivity: < 0.1 % with human Collagen I, II, III, IV, V, human Fibronectin, or rat Elastin by radioimmunoassay.

Purification: Protein A purified

Immunogen: Elastin extracted and purified from human skin

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, IF (1:80), IHC, IHC-P (2.5 μg/mL), RIA

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Immunofluorescence: 1:80. Frozen human skin and liver tissues. Positive control: Human skin or liver.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS buffer,  pH 7.2 with no preservatives added

Preservative: Without preservative

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: ELN / Elastin (ELN Products)

Synonyms: SVAS antibody, WBS antibody, WS antibody, AI385707 antibody, AI480567 antibody, E030024M20Rik antibody, RATTREL11 antibody, TREL11 antibody, Trela antibody, Trela26 antibody, ELN antibody, elastin antibody, ELN antibody, Eln antibody, LOC574132 antibody, Tcur_2481 antibody, Kfla_2289 antibody, LOC100620140 antibody

Background: Name/Gene ID: ELN

Synonyms: ELN, SVAS, WBS, Elastin, Tropoelastin, WS

Gene ID: 2006

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization