p53 antibody

Catalog No. ABIN306557

The Sheep Polyclonal anti-p53 antibody is suitable to detect p53 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IP.

Quick Overview for p53 antibody (ABIN306557)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human, Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-p53 Antibody

Purpose: Sheep anti Human p53

Characteristics: P53 is a transcription factor with a molecular weight of 53 kDa that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein.

Purification: Ammonium Sulfate Precipitation

Isotype: IgG

Application Details

Application Notes: Applications: Western Blot (1-10 μg/mL), Immunoprecipitation, immunostaining of formalin-fixed and paraffine embedded tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Molecular Weight: 53kDa

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect