TSC1 antibody (Internal Region)

Catalog No. ABIN307592

Product Details anti-TSC1 Antibody

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSC1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes an epitope within the internal region of the 130kD tumor suppressor protein, Tumour sclerosis complex 1 (TSC1). Mutation within the TSC1 gene is responsible for Tuberous sclerosis (TSC), an autosomal dominant genetic disorder associated with the development of benign tumors (hamartomas), a condition also induced by mutation of the Tumour sclerosis complex 2 (TSC2) gene. Many proteins have been shown to play a role in the regulation of the serine/threonine kinase known as target of rapamycin (TOR), a central component in a complex signaling pathway which controls cell proliferation and cell cycle progression. Negative regulation of TOR activity occurs following the over-expression of the TSC1 and TSC2 gene products, hamartin and tuberin. These act by suppressing the phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K), downstream targets of TOR involved in mRNA translation. Furthermore the TOR activator protein Rheb has been identified as a target of TSC1-TSC2 complexes. Has been reported as suitable for use in IHC on mouse EL4 cell cytospins at 2 μg/mL.

Purification: Purified

Immunogen: A 15 amino acid peptide located near the center of human TSC1.

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: LS-C42321 detects a band of approximately 130kD in mouse EL4 °Cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Alternative Name: Hamartin / TSC1 (TSC1 Products)

Synonyms: LAM antibody, TSC antibody, hamartin antibody, mKIAA0243 antibody, tsc1 antibody, wu:fc38b04 antibody, fa99f04 antibody, wu:fa99f04 antibody, zgc:63657 antibody, TSC complex subunit 1 antibody, tuberous sclerosis 1 antibody, TSC complex subunit 1b antibody, TSC complex subunit 1a antibody, TSC1 antibody, Tsc1 antibody, tsc1b antibody, tsc1 antibody, tsc1a antibody

Background: Name/Gene ID: TSC1

Synonyms: TSC1, LAM, Tuberous sclerosis 1 protein, Tuberous sclerosis 1, Hamartin, KIAA0243, TSC, Tumor suppressor

Gene ID: 7248

UniProt: Q92574

Pathways: RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation