Caspase 3 antibody
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- Target See all Caspase 3 (CASP3) Antibodies
- Caspase 3 (CASP3)
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Reactivity
- Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Caspase 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- The antibody detects endogenous Active Caspase-3 protein.
- Characteristics
- Mouse Monoclonal to Active Caspase-3.
- Purification
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Recombinant Protein
- Clone
- 5E1
- Isotype
- IgG1
- Top Product
- Discover our top product CASP3 Primary Antibody
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- Application Notes
- WB 1:500-1000, IHC 1:100-200
- Restrictions
- For Research Use only
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- by
- Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #100061
- Date
- 08/31/2016
- Antigen
- Caspase 3 (active)
- Lot Number
- Method validated
- Western Blotting
- Positive Control
- Mouse cerebellar tissue samples at post natal day P5
- Negative Control
- Notes
- The antibody recognized both the active and non-active form of Caspase3 and worked well in the experimental conditions tested. The product is therefore indicated for the WB use.
- Primary Antibody
- Secondary Antibody
- ABIN3179097
- Full Protocol
- Mouse cerebellar tissue samples at post natal day (P) 5 were lysed in 150 µL of lysis buffer.
- Protein concentration was determined using Bradford reagen (Sigma B6916) according to the manufacturer's recommendation.
- 30 and 60µg total protein were denatured in sample buffer for 5min at 95°C and then cooled on ice.
- Proteins were separated on a denaturing homemade 15% SDS-PAGE gel at a constant voltage of 180V for 70min.
- Proteins were transferred in a transfer buffer to nitrocellulose membrane (Amersham Biosciences, RPN203D, lot 18100/66/0207) at constant amperage of 400mA for 60min.
- Blocking of the membrane with blocking solution (0.01M PBS, 0.1% tween, 2% Bovine Serum Albumin) for 1h at RT.
- Incubation with the primary antibody ABIN3179097 diluted 1:1000 in blocking solution ON at 4°C.
- 3 washes in PBS-0.1% tween for 15min at RT.
- Incubation with the anti-mouse horseradish peroxidase (HRP) secondary antibody (Santa Cruz, SC2005, lot H0406) diluted 1:10000 in blocking solution for 1h at RT.
- Washes in PBS-0.1% tween for 15min at RT.
- Chemiluminescence detection using homemade ECL (1min incubation at RT) and revealed on HYPERFILM EGL (Amersham Biosciences, RPN2013K, lot 04450300115); exposure 30sec.
- Proteins were revealed using the ECL technique on western blot films for 30 seconds.
- Experimental Notes
- A band around 30-35 kDa and a band around 17kDa were detected with the experimental procedures previously described.
- A protein load of 30µg is recommended, as in the 60µg load, weak non-specific bands were detected around 15 and 30kDa.
Validation #100061 (Western Blotting)Validation ImagesFull Methods -
- Format
- Liquid
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C.
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-
Alpha-1-Antitrypsin Protects Vascular Grafts of Brain-Dead Rats Against Ischemia/Reperfusion Injury." in: The Journal of surgical research, Vol. 283, pp. 953-964, (2023) (PubMed).
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Alpha-1-Antitrypsin Protects Vascular Grafts of Brain-Dead Rats Against Ischemia/Reperfusion Injury." in: The Journal of surgical research, Vol. 283, pp. 953-964, (2023) (PubMed).
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- Target
- Caspase 3 (CASP3)
- Alternative Name
- Caspase-3 (CASP3 Products)
- Synonyms
- CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody
- Gene ID
- 836
- UniProt
- P42574
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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