EpCAM antibody

Catalog No. ABIN319521

Product Details anti-EpCAM Antibody

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the 40 kDa cell surface antigen known as CD326.

Clone: MOC-31

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 10 μg/mL diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (10 μg/mL). Immunohistochemistry on Paraffin Sections: This antibody requires protein digestionpre-treatment of paraffin sections e. g. trypsin or pronase. Antigen retrieval is requiredusing heat treatment prior to staining of paraffin sections. Recommended Positive Control Tissue: Lung carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, Azide Free, None, None

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: CD326 / EPCAM / TACSTD1 (EPCAM Products)

Synonyms: DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody

Background: Lung cancer is a heterogeneous disease, which can be subdivided into five major tyoes based on histopathological criteria like squamous cell carcinomas, adenocarcinoma, large cell carcinoma, adenosquamous carcinoma and small cell lung cancer (SCLC). A number of important prognostic characteristics have been identified to include the extend of disease and especially for SCLC, tumor sensitivity to chemotherapy. Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Cell Adhesion Molecule (Ep-CAM), Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against CD326, many of which have been described as tumour specific molecules on carcinomas. CD326 is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1

Gene ID: 4072

NCBI Accession: NP_002345

UniProt: P16422