IKK alpha antibody (Biotin)

Catalog No. ABIN3197521

The Mouse Monoclonal anti-IKK alpha antibody has been validated for WB and IP. It is suitable to detect IKK alpha in samples from Human, Mouse and Monkey.

Quick Overview for IKK alpha antibody (Biotin) (ABIN3197521)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKK alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-IKK alpha Antibody (Biotin)

Specificity: Recognizes human IKK alpha. Species cross-reactivity: mouse and monkey.

Purification: Purified

Immunogen: His-tagged full-length human IKKa/IKK1 protein.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: IP (1 - 2 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in Western Blot and immunoprecipitation. Immunoprecipitation: 1-2 μg/mL. Western Blot: 1 μg/mL. Detects a band at ~85kD. Positive control: Daudi, NIH 3T3, or Raw cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide. Labeled, Biotin

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: CHUK / IKKA / IKK Alpha

Background: Name/Gene ID: CHUK
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: CHUK, I-kappa-B kinase alpha, I-kappa-B kinase-alpha, Ikb kinase alpha, IKK-A, IKBKA, IKK1, NFKBIKA, I-kappa-B kinase 1, IkappaB kinase, IKK Alpha, IKK-alpha, IKKA, Transcription factor 16, IkappaBalpha Kinase, IkB kinase alpha subunit, IKK-a kinase, Ikkalpha, TCF-16, TCF16

Gene ID: 1147

UniProt: O15111

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins