TRAIL antibody (AA 114-281)

Catalog No. ABIN3201326

Product Details anti-TRAIL Antibody

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Binding Specificity: AA 114-281

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAIL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

Sequence: MGHHHHHHSG SEF-VRERGPQ RVAAHITGTR GRSNTLSSPN SKNEKALGRK INSWESSRSG HSFLSNLHLR NGELVIHEKG FYYIYSQTYF RFQEEIKENT KNDKQMVQYI YKYTSYPDPI LLMKSARNSC WSKDAEYGLY SIYQGGIFEL KENDRIFVSV TNEHLIDMDH EASFFGAFLV G

Specificity: The antibody is a mouse monoclonal antibody raised against TRAIL. It has been selected for its ability to recognize TRAIL in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) corresdonding to Val114~Gly281 with N-terminal His Tag

Clone: 18#

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TNFSF10 Products)

Synonyms: APO2L antibody, Apo-2L antibody, CD253 antibody, TL2 antibody, TRAIL antibody, A330042I21Rik antibody, AI448571 antibody, APO-2L antibody, Ly81 antibody, Trail antibody, tl2 antibody, apo2l antibody, cd253 antibody, trail antibody, apo-2l antibody, trail1 antibody, xtrail1 antibody, tnfsf10l2 antibody, zgc:92320 antibody, TNLG6A antibody, TNF superfamily member 10 antibody, tumor necrosis factor (ligand) superfamily, member 10 antibody, tumor necrosis factor superfamily member 10 antibody, TNF superfamily member 10 S homeolog antibody, TNFSF10 antibody, Tnfsf10 antibody, tnfsf10.S antibody, tnfsf10 antibody

Background: CD253, TNFSF10, APO2L, Apo2-L, TL2, TRAIL, Tumor Necrosis Factor Ligand Superfamily Member 10, Apo-2 Ligand

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity