Notch1 antibody (AA 2428-2556)
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- Target See all Notch1 (NOTCH1) Antibodies
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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Binding Specificity
- AA 2428-2556
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Reactivity
- Human
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This Notch1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificity
- Recognizes human Notch-1 intracellular domain. Species sequence homology: canine 83 % and mouse 89 %. < 1 % cross-reactivity with rhNotch-2 IC, rhNotch-3 IC, and rhNotch-4 IC.
- Purification
- Affinity purified
- Immunogen
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E. coli-derived recombinant human rhNotch-1 (aa 2428 - 2556).
Type of Immunogen: Recombinant protein - Isotype
- IgG
- Top Product
- Discover our top product NOTCH1 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in for use in Western blot, Immunocytochemistry, Flow cytometry. and Direct ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit for rhNotch-1 is ~0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Notch-1 in SAOS human osteosarcoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for. 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Flow cytometry: 50 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding 10il of a 25 μg/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Notch-1. The detection limit is ~0.2 ng/well. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute, 0.5 mL sterile PBS to a concentration 0.2 mg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose. Reconstitute, 0.5 mL sterile PBS to a concentration 0.2 mg/mL.
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- Target
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- Alternative Name
- NOTCH1 (NOTCH1 Products)
- Synonyms
- notch1 antibody, NOTCH1 antibody, hn1 antibody, tan1 antibody, notch antibody, xotch antibody, xnotch antibody, notch-1 antibody, xnotch1 antibody, TAN1 antibody, hN1 antibody, 9930111A19Rik antibody, Mis6 antibody, N1 antibody, Tan1 antibody, lin-12 antibody, NOTCH antibody, Xotch antibody, notch1-a antibody, notch 1 antibody, fibropellin-1 antibody, Notch homolog 1, translocation-associated (Drosophila) antibody, Notch homolog 1, translocation-associated antibody, notch 1 S homeolog antibody, notch1 antibody, LOC100631526 antibody, NOTCH1 antibody, Notch1 antibody, notch1.S antibody
- Background
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Name/Gene ID: NOTCH1
Synonyms: NOTCH1, Notch 1, TAN1 - Gene ID
- 4851
- UniProt
- P46531
- Pathways
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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