PPP2CA antibody (AA 209-309)

Catalog No. ABIN325661

Product Details anti-PPP2CA Antibody

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Binding Specificity: AA 209-309

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This PPP2CA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes human PP2A. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the antigen, it is expected that both isoforms will be detected by the antibody.

Purification: Protein G purified

Immunogen: E. coli-derived recombinant human PP2A-alpha catalytic subunit (aa209-309).

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IF, IHC, IHC-P

Usage: Immunohistochemistry. Immunohistochemistry: This antibody was tested on paraffin-embedded human tissues (liver, small intestine and colon cancer). Recommended working dilution is 8-25 μg/mL when using an HRP-DAB detection system. Positive control: Normal human liver. Antibody diluent: 0.1 M PBS (  pH 7.4) containing 1 % bovine serum albumin, 1 % normal donkey serum, 0.3 % Triton X-100, and 0.01 % sodium azide. Antigen retrieval: Is not usually required for this target protein, but if low intensity of tissue staining is observed, heat-induced epitope retrieval of PP2A antigen can be done. Staining pattern: Many PP2A-immunoreactive hepatocytes can be found in normal human liver. PP2A staining can be seen in the cytoplasm and has punctate appearance.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Alternative Name: PP2Ac / PPP2CA (PPP2CA Products)

Synonyms: PP2Ac antibody, PP2CA antibody, PP2Calpha antibody, RP-C antibody, PP2A antibody, R75353 antibody, Pp2a1 antibody, pp2ac antibody, zgc:110641 antibody, protein phosphatase 2 catalytic subunit alpha antibody, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform antibody, protein phosphatase 2, catalytic subunit, alpha isozyme antibody, protein phosphatase 2 catalytic subunit alpha L homeolog antibody, protein phosphatase 2, catalytic subunit, beta isozyme antibody, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody, PPP2CA antibody, Ppp2ca antibody, ppp2ca.L antibody, ppp2cb antibody, LOC471633 antibody, ppp2ca antibody

Background: Name/Gene ID: PPP2CA
Subfamily: PP2A
Family: Protein Phosphatase

Synonyms: PPP2CA, PP2Ac, PP2Calpha, ppp2A1, Protein phosphatase 2A alpha, Replication protein C, PP2CA, RP-C, PP2A-alpha

Gene ID: 5515

UniProt: P67775

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades