Ret Proto-Oncogene antibody (AA 29-635)
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- Target See all Ret Proto-Oncogene (RET) Antibodies
- Ret Proto-Oncogene (RET)
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Binding Specificity
- AA 29-635
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Ret Proto-Oncogene antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- Specificity
- Recognizes human Ret. Species cross-reactivity: Mouse.
- Purification
- Protein G purified
- Immunogen
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Recombinant protein corresponding to aa29-635 from human Ret/Fc Chimera expressed in Sf21 cells (P07949).
Type of Immunogen: Recombinant protein - Isotype
- IgG1
- Top Product
- Discover our top product RET Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 μg/mL, For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Western Blot: 1-2 μg/mL, Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhRet and rmRet is ~6 ng/well. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with sterile PBS to a concentration of 0.5 mg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose.
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
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- Target
- Ret Proto-Oncogene (RET)
- Alternative Name
- RET (RET Products)
- Synonyms
- c-ret antibody, cret antibody, etID315074.13 antibody, ret1 antibody, wu:fd13h01 antibody, X-ret antibody, ret-A antibody, xret antibody, RET antibody, MTC1 antibody, CDHF12 antibody, CDHR16 antibody, HSCR1 antibody, MEN2A antibody, MEN2B antibody, PTC antibody, RET-ELE1 antibody, RET51 antibody, RET9 antibody, c-Ret antibody, ret proto-oncogene receptor tyrosine kinase antibody, ret proto-oncogene S homeolog antibody, ret proto-oncogene antibody, ret antibody, ret.S antibody, RET antibody, Ret antibody
- Background
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Name/Gene ID: RET
Subfamily: Cadherin
Family: Protein Kinase
Synonyms: RET, Cadherin family member 12, CDHF12, C-ret, Hirschsprung disease 1, HSCR1, Hydroxyaryl-protein kinase, Oncogene ret, Proto-oncogene c-Ret, RET-ELE1, RET51, Ret proto-oncogene, RET transforming sequence, RET43, Men2-ret, MEN2B, MTC1, Receptor tyrosine kinase, CDHR16, MEN2A, PTC, RET9 - Gene ID
- 5979
- UniProt
- P07949
- Pathways
- RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation
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