SMAD1 antibody

Catalog No. ABIN325894

Product Details anti-SMAD1 Antibody

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity: Recognizes human Smad1.

Purification: Protein G purified

Immunogen: Recombinant human Smad1.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (0.5 - 2 μg/mL), ICC (2 - 100 μg/mL), IP, WB (0.2 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Flow Cytometry: 0.5-2 μg/mL. HeLa cells were fixed with 2 % paraformaldehyde in 90 % methanol. Western Blot: 0.2-2 μg/mL. Immunocytochemistry: 2-100 μg/mL. HeLa cells were fixed with 4 % paraformaldehyde and permeabilized with 0.1 % Triton X-100 in PBS. Immunoprecipitation: 100-500 μg/sample.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1 (SMAD1 Products)

Synonyms: MLP antibody, Xmad1 antibody, Xsmad1 antibody, bsp1 antibody, jv4-1 antibody, jv41 antibody, madh1 antibody, madr1 antibody, smad-1 antibody, MADH1 antibody, fb39h09 antibody, wu:fb39h09 antibody, wu:fb97e04 antibody, 2/23 antibody, CG12399 antibody, Dmel\\CG12399 antibody, E(zen)2 antibody, En(vvl) antibody, MAD antibody, Mat antibody, P-Mad antibody, P-mad antibody, PMad antibody, Smad antibody, Smad1 antibody, apg antibody, c28 antibody, dMAD antibody, dMad antibody, l(2)K00237 antibody, l(2)k00237 antibody, mad antibody, p-Mad antibody, pMAD antibody, pMad antibody, pSmad antibody, BSP-1 antibody, BSP1 antibody, JV4-1 antibody, JV41 antibody, MADR1 antibody, AI528653 antibody, Madh1 antibody, Madr1 antibody, Mlp1 antibody, MusMLP antibody, smad1 antibody, xmad antibody, SMAD family member 1 antibody, Mothers against dpp antibody, SMAD family member 1 L homeolog antibody, smad1 antibody, SMAD1 antibody, Mad antibody, Smad1 antibody, smad1.L antibody

Background: Name/Gene ID: SMAD1

Synonyms: SMAD1, BSP1, BSP-1, JV4-1, MADH1, Mothers against DPP homolog 1, JV41, SMAD family member 1, TGF-beta signaling protein 1, MADR1, HSMAD1, MAD homolog 1, Mad-related protein 1, SMAD 1

Gene ID: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development