TGFBR3 antibody (Extracellular Domain)

Catalog No. ABIN326173

Product Details anti-TGFBR3 Antibody

Target: TGFBR3 (Transforming Growth Factor, beta Receptor III (TGFBR3))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TGFBR3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Specificity: Recognizes human TGFb RIII. Based on direct ELISA and Western Blot results, this antibody shows less than 1 % cross-reactivity with rhTGFb RII.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rhTGFb sRIII extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human TGF- RIII. The detection limit for rhTGF- RIII is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Tested in flow cytometry using HeLa cells. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human TGF- RIII. The detection limit for rhTGF- RIII is approximately 0.16 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1.25 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose.

Storage: -20 °C

Storage Comment: Reconstitute with sterile PBS and store at -20°C. Aliquot to avoid freeze-thaw cycles.

Target Details for TGFBR3

Target: TGFBR3 (Transforming Growth Factor, beta Receptor III (TGFBR3))

Alternative Name: Betaglycan / TGFBR3 (TGFBR3 Products)

Synonyms: TGFBR3 antibody, DKFZp469I0915 antibody, BGCAN antibody, betaglycan antibody, 1110036H20Rik antibody, AU015626 antibody, AW215636 antibody, TBRIII antibody, transforming growth factor beta receptor 3 antibody, transforming growth factor, beta receptor III antibody, TGFBR3 antibody, tgfbr3 antibody, Tgfbr3 antibody

Background: Name/Gene ID: TGFBR3

Synonyms: TGFBR3, Betaglycan, BGCAN, TGF-beta receptor type 3, TGF-beta receptor type III, TGFR-3, Betaglycan proteoglycan, Tgf-beta receptors type iii

Gene ID: 7049

UniProt: Q03167