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MBD2 antibody (Internal Region)

Verified MBD2 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN334432
  • Target See all MBD2 Antibodies
    MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
    Binding Specificity
    • 9
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 47
    • 23
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 45
    • 2
    Goat
    Clonality
    • 44
    • 3
    Polyclonal
    Conjugate
    • 25
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MBD2 antibody is un-conjugated
    Application
    • 40
    • 21
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    MBD2 (isoform 1)
    Sequence
    RNDPLNQNKG KPDLN
    Specificity
    This antibody is expected to resognize isoform 1 (NP_003918.1) only.
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bartels, Spruijt, Brinkman, Jansen, Vermeulen, Stunnenberg: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).

    Spruijt, Bartels, Brinkman, Tjeertes, Poser, Stunnenberg, Vermeulen: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." in: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).

  • Target
    MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
    Alternative Name
    MBD2 (MBD2 Products)
    Synonyms
    MBD2 antibody, DMTase antibody, NY-CO-41 antibody, MBD2a antibody, wu:fa97f06 antibody, zgc:111862 antibody, methyl-CpG binding domain protein 2 antibody, methyl-CpG binding domain protein 2 S homeolog antibody, MBD2 antibody, Mbd2 antibody, mbd2 antibody, mbd2.S antibody
    Background
    MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41
    Gene ID
    8932, 17191
    NCBI Accession
    NP_003918
    Pathways
    Chromatin Binding
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