Keratin 10/13 antibody

Catalog No. ABIN335338

Product Details

Target: Keratin 10/13

Reactivity: Human, Cat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Human and feline.

Purification: Purified

Immunogen: DE-K13 is a mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 mouse immunized with a cytoskeletal preparation extracted from human ectocervical epithelium.

Clone: DE-K13

Isotype: IgG2a

Application Details

Application Notes: DE-K13 reacts with cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. Cytokeratin 10 is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while cytokeratin 13 is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia, as well as carcinomas derived from these tissues. DE-K13 is useful for immunoblotting, immunocytochemistry, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues (see also above). For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1N HCl for 30 min at room temperature is required. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

van Bommel, Kenemans, Helmerhorst, Gallee, Ivanyi: "Expression of cytokeratin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix." in: Cancer, Vol. 74, Issue 8, pp. 2314-20, (1994) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard, Misdorp: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." in: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, (1993) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, (1992) (PubMed).

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10+13 / Keratin K10+K13

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.