NR4A1 antibody (AA 401-414)
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- Target See all NR4A1 Antibodies
- NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))
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Binding Specificity
- AA 401-414
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Reactivity
- Human, Mouse, Rat, Dog, Horse, Rabbit, Hamster
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NR4A1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NR4A1 / NUR77. This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%) Marmoset, Bat (93%) Bovine (86%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-GRLPSKPKQPPDAS from an internal region of human NR4A1 / NUR77 (NP_002126.2, NP_775180.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%), Marmoset, Bat (93%), Bovine (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR4A1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))
- Alternative Name
- NR4A1 / NUR77 (NR4A1 Products)
- Synonyms
- GFRP1 antibody, HMR antibody, N10 antibody, NAK-1 antibody, NGFIB antibody, NP10 antibody, NUR77 antibody, TR3 antibody, NGFI-B antibody, Gfrp antibody, Hbr-1 antibody, Hbr1 antibody, Hmr antibody, TIS1 antibody, nur77 antibody, Ngfi-b antibody, Nur77 antibody, nuclear receptor subfamily 4 group A member 1 antibody, nuclear receptor subfamily 4, group A, member 1 antibody, NR4A1 antibody, Nr4a1 antibody
- Background
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Name/Gene ID: NR4A1
Subfamily: NR4 Nerve growth factor-like
Family: NHR
Synonyms: NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, Orphan nuclear receptor HMR - Gene ID
- 3164
- NCBI Accession
- NP_002126, NP_775180
- UniProt
- P22736
- Pathways
- Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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