P2RY1 antibody (AA 345-356)

Catalog No. ABIN342989

Product Details anti-P2RY1 Antibody

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Binding Specificity: AA 345-356

Reactivity: Human, Cow, Guinea Pig, Rabbit, Dog, Bat, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This P2RY1 antibody is un-conjugated

Application: ELISA

Specificity: Human P2RY1 / P2Y1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Opossum, Guinea pig (100%) Mouse, Rat, Hamster, Horse (92%) Lizard, Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RSEANLQSKSED from the C-terminus of human P2RY1 / P2Y1 (NP_002554.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Opossum, Guinea pig (100%), Mouse, Rat, Hamster, Horse (92%), Lizard, Xenopus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:8000)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments gave an approx. 50kD band in lysates of Human Lung and of cell line A549 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 42.1kD according to NP_002554.1. The 50kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for P2RY1

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Alternative Name: P2RY1 / P2Y1 (P2RY1 Products)

Synonyms: P2Y1 antibody, P2y antibody, P2y1 antibody, purinergic receptor P2Y, G-protein coupled 1 antibody, purinergic receptor P2Y1 antibody, P2ry1 antibody, P2RY1 antibody

Background: Name/Gene ID: P2RY1
Subfamily: Purinergic
Family: GPCR

Synonyms: P2RY1, ATP receptor, p2Y1, p2y1r, Platelet ADP receptor, Purinoceptor p2y1, Purinergic receptor, p2 purinoceptor subtype Y1, p2RY1, p2Y purinoceptor 1, p2y-1-r, Purinoceptor p2yr1, p2y1 receptor

Gene ID: 5028

NCBI Accession: NP_002554

UniProt: P47900

Pathways: Regulation of Carbohydrate Metabolic Process, Feeding Behaviour