ATP7A antibody (AA 1212-1225)

Catalog No. ABIN343024

Product Details anti-ATP7A Antibody

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Binding Specificity: AA 1212-1225

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP7A antibody is un-conjugated

Application: ELISA

Specificity: Human ATP7A.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%) Gorilla (93%) Marmoset, Panda, Bovine, Horse (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HKNNWNIEDNNIKN from an internal region of human ATP7A (NP_000043.3). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%), Gorilla (93%), Marmoset, Panda, Bovine, Horse (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:8000)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments gave an approx 55kD band in Human Colon lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 163kD according to NP_000043.3. The 55kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATP7A

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Alternative Name: MNK / ATP7A (ATP7A Products)

Synonyms: ATP7A antibody, cal antibody, wu:fc43e01 antibody, zgc:153422 antibody, zgc:158633 antibody, DDBDRAFT_0218568 antibody, DDBDRAFT_0235190 antibody, DDB_0218568 antibody, DDB_0235190 antibody, atpase antibody, Atp7a antibody, kal antibody, atp7a antibody, DSMAX antibody, MK antibody, MNK antibody, SMAX3 antibody, Blo antibody, DXHXS1608e antibody, I14 antibody, Mo antibody, blotchy antibody, br antibody, brindled antibody, mottled antibody, Mnk antibody, ATPase copper transporting alpha antibody, ATPase, Cu++ transporting, alpha polypeptide antibody, P-type ATPase antibody, ATP synthase subunit a antibody, copper-transporting ATPase 1 antibody, ATP7A antibody, atp7a antibody, LOC100049514 antibody, Atp7a antibody, LOC412379 antibody

Background: Name/Gene ID: ATP7A
Subfamily: ATPase - P type, type IB
Family: Transporter

Synonyms: ATP7A, Copper pump 1, Menkes disease ATPase, MNK, MK, SMAX3, Menkes syndrome, OHS, Copper-transporting ATPase 1, DSMAX, MC1

Gene ID: 538

NCBI Accession: NP_000043

UniProt: Q04656

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process