GFP antibody

Catalog No. ABIN356346

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Characteristics: Synonyms: Green fluorescent protein, GFP-Tag

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Clone: 168AT1211

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/3,000. Western blotting: 1/2,000. Immunohistochemistry: 1/50-1/100. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-GFP antibody (ABIN356346)

Hanna, Bednářová, Rakshit, Chaudhuri, ODonnell, Krishnan: "Perturbations in dopamine synthesis lead to discrete physiological effects and impact oxidative stress response in Drosophila." in: Journal of insect physiology, Vol. 73, pp. 11-9, (2015) (PubMed).

Fekete, Rojewski, Fürst, Kreja, Ignatius, Dausend, Schrezenmeier: "GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC." in: PLoS ONE, Vol. 7, Issue 8, pp. e43255, (2012) (PubMed).

Nicholls, Chu, Abbruzzese, Tremblay, Hardy: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, (2011) (PubMed).

Clark, Jovov, Rooj, Fuller, Benos: "Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27130-43, (2010) (PubMed).

Maier, Ogihara, Trace, Tersey, Robbins, Chakrabarti, Nunemaker, Stull, Taylor, Thompson, Dondero, Lewis, Dinarello, Nadler, Mirmira: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, Vol. 120, Issue 6, pp. 2156-70, (2010) (PubMed).

Kim, Nadal, Clemens, Baron, Jung, Misumi, Rudy, Hoffman: "Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons." in: Journal of neurophysiology, Vol. 100, Issue 4, pp. 1835-47, (2008) (PubMed).

Witola, Pessi, El Bissati, Reynolds, Mamoun: "Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 21305-11, (2006) (PubMed).

Stomski, Woodcock, Zacharakis, Bagley, Sun, Lopez et al.: "Identification of a Cys motif in the common beta chain of the interleukin 3, granulocyte-macrophage colony-stimulating factor, and interleukin 5 receptors essential for disulfide-linked receptor ..." in: The Journal of biological chemistry, Vol. 273, Issue 2, pp. 1192-9, (1998) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Target Type: Viral Protein

Background: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunoprecipitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.Synonyms: GFP-Tag, Green fluorescent protein

UniProt: P42212