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DYKDDDDK Tag antibody
WB, EIA
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-DYKDDDDK Tag Antibody
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Target
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DYKDDDDK Tag
Reactivity
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Host
All hosts for DYKDDDDK Tag antibodies
Rabbit
Clonality
All clonalities for DYKDDDDK Tag antibodies
Polyclonal
Conjugate
All conjugates for DYKDDDDK Tag antibodies
This DYKDDDDK Tag antibody is un-conjugated
Application
All applications for DYKDDDDK Tag antibodies
Western Blotting (WB), Enzyme Immunoassay (EIA)
Sequence
DYKDDDDK
Purification
Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen
KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Isotype
Ig Fraction
Alternatives
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Application Details
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Application Notes
ELISA: 1/5,000. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS containing 0.09 % (w/v) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target Details for DYKDDDDK Tag
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Target
DYKDDDDK Tag
Alternative Name
DYKDDDDK Epitope Tag (DYKDDDDK Tag Products )
Target Type
Tag
Background
Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1).
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