PDK2 antibody (C-Term)

Catalog No. ABIN359096

The Rabbit Polyclonal anti-PDK2 antibody is suitable to detect PDK2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for PDK2 antibody (C-Term) (ABIN359096)

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PDK2 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to PDK2.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human PDK2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PDK2

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Alternative Name: PDK2

Background: PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.Synonyms: Pyruvate dehydrogenase kinase isoform 2, Pyruvate dehydrogenase lipoamide kinase isozyme 2 mitochondrial

Gene ID: 5164, 9606

UniProt: Q15119

Pathways: PI3K-Akt Signaling, RTK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect