The antibody detects endogenous level of total Akt protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Specificity/Sensitivity Akt (Ab-308) antibody detects endogenous levels of total Akt protein.
Immunogen
Peptide sequence around AA 306-310 (M-K-T-F-C) derived from Human AKT1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
AKT1
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Li, Lu, Chen, Bu, Huang: "Loss of fatty acid synthase inhibits the "HER2-PI3K/Akt axis" activity and malignant phenotype of Caco-2 cells." in: Lipids in health and disease, Vol. 12, pp. 83, (2013) (PubMed).
Zhang, Hou, Cui, Guo, Shi, Yang, Dai: "Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity." in: Toxicology letters, Vol. 218, Issue 1, pp. 61-9, (2013) (PubMed).
Li, Bu, Wu, Wu, Huang: "The "HER2-PI3K/Akt-FASN Axis" regulated malignant phenotype of colorectal cancer cells." in: Lipids, Vol. 47, Issue 4, pp. 403-11, (2012) (PubMed).
Guo, Yang, Lu, Li, Wan, Long, Li, Feng, Li: "Rb1 postconditioning attenuates liver warm ischemia-reperfusion injury through ROS-NO-HIF pathway." in: Life sciences, Vol. 88, Issue 13-14, pp. 598-605, (2011) (PubMed).
Ban, Uno, Nakamura: "Suppression of hypoxia-induced HIF-1alpha accumulation by VEGFR inhibitors: Different profiles of AAL993 versus SU5416 and KRN633." in: Cancer letters, Vol. 296, Issue 1, pp. 17-26, (2010) (PubMed).
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.