GRP78 antibody (Internal Region)
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- Target See all GRP78 (HSPA5) Antibodies
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
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Binding Specificity
- AA 250-300, Internal Region
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Reactivity
- Human, Rat, Mouse, Drosophila melanogaster, Sheep, Chicken
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GRP78 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- Specificity
- Reacts with GRP-78 protein from human, mouse, rat, Drosophila, sheep and chicken
- Cross-Reactivity (Details)
- Predicted to cross reacts with proteins from Bovine, C elegans, Primate, Zebrafish due to 100 % sequence homology.
- Purification
- Antigen affinity purified
- Immunogen
- Synthetic peptide derived from internal domain of the human Grp78 protein. Within residues 250-300.
- Top Product
- Discover our top product HSPA5 Primary Antibody
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- Application Notes
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Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(0.5 μg/mL) FC(1:50) ICC/ IF(1:50) - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.67 mg/mL
- Buffer
- PBS, glycerol 30 % , Sodium azide 0.1 %
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Hu, Mu, Rt, Ch, Dr, Sh, Bv, Ce, Pm, Ze ovine (100 % ), Zebrafish (100 %), Primate (100 %), C. elegans (100 %).
- Storage
- 4 °C/-20 °C
- Storage Comment
- Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
- Expiry Date
- 6 months
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- Target
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- Alternative Name
- 78 kDa Glucose-Regulated Protein (GRP-78) (HSPA5 Products)
- Synonyms
- GRP78 antibody, BiP antibody, GRP-78 antibody, grp78 antibody, hspa5a antibody, BIP antibody, MIF2 antibody, AL022860 antibody, AU019543 antibody, Bip antibody, D2Wsu141e antibody, D2Wsu17e antibody, Grp78 antibody, Hsce70 antibody, SEZ-7 antibody, Sez7 antibody, baffled antibody, mBiP antibody, cb865 antibody, fb60h09 antibody, fi36d04 antibody, wu:fb60h09 antibody, wu:fi36d04 antibody, zgc:55994 antibody, zgc:77606 antibody, 78 kDa glucose-regulated protein antibody, heat shock protein family A (Hsp70) member 5 antibody, BiP/GRP78 antibody, glucose-regulated protein 78 antibody, putative glucose-regulated protein 78 antibody, Hsp70 family ATPase KAR2 antibody, heat shock protein family A (Hsp70) member 5 S homeolog antibody, heat shock 70 kDa protein 5a antibody, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody, heat shock protein 5 antibody, heat shock protein family A member 5 antibody, CpipJ_CPIJ003550 antibody, HSPA5 antibody, grp78 antibody, LOC100533358 antibody, BiP/grp78 antibody, Tc00.1047053506585.40 antibody, Tb11.02.5450 antibody, Tb11.02.5500 antibody, LMJF_28_1200 antibody, KAR2 antibody, LOC100135840 antibody, hspa5.S antibody, hspa5 antibody, Hspa5 antibody
- Background
- Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimer's disease patients.
- Gene ID
- 3309
- Pathways
- Thyroid Hormone Synthesis, ER-Nucleus Signaling
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