FOXO3 antibody (AA 600-673)

Catalog No. ABIN372285

This anti-FOXO3 antibody is a Rabbit Polyclonal antibody detecting FOXO3 in WB and IHC (p). Suitable for Human, Mouse, Rat, Dog, Cow, Chicken, Monkey and Xenopus laevis.

Quick Overview for FOXO3 antibody (AA 600-673) (ABIN372285)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO3 Antibody

Binding Specificity: AA 600-673

Specificity: This antibody recognizes Forkhead Box O3 (FOXO3)

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Chicken, Dog (Canine), Xenopus, Monkey, Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a portion of the amino acids 600-673 of human FOXO3A.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 10 μg/mL. Western Blot: 2-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % Sodium Azide as preservative and 0.05 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3 / FKHRL1

Background: FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation. FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. FOXO3a has been shown to induce cell growth arrest to effect tumor suppression and FOXO3A genotype is strongly associated with human longevity.Synonyms: AF6q21 protein, FOXO3A, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1

Molecular Weight: 71 kDa.

Gene ID: 2309

NCBI Accession: NP_001446

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis