ARPC3 antibody (C-Term)

Catalog No. ABIN374430

Product Details anti-ARPC3 Antibody

Target: ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-RQFMNKSLSG PGQ

Specificity: This antibody recognizes ARPC3.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human and Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_005710.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 1-3 μg/mL. Band observed just below 20 kDa marker in Hela, Human Brainand Mouse Brain lysates (predicted MW of 20 kDa according to NP_005710).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARPC3

Target: ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))

Alternative Name: ARPC3 / ARC21 (ARPC3 Products)

Synonyms: ARC21 antibody, p21-Arc antibody, 1110006A04Rik antibody, 21kDa antibody, AA408672 antibody, AI788639 antibody, p21-Ar antibody, p21Arc antibody, arc21 antibody, p21-arc antibody, zgc:86828 antibody, actin related protein 2/3 complex subunit 3 antibody, actin related protein 2/3 complex, subunit 3 antibody, actin related protein 2/3 complex subunit 3 L homeolog antibody, actin related protein 2/3 complex, subunit 3, 21kDa antibody, actin related protein 2/3 complex subunit 3 pseudogene antibody, actin-related protein 2/3 complex subunit 3 antibody, actin-related protein 2/3 complex subunit 3 pseudogene antibody, ARPC3 antibody, Arpc3 antibody, arpc3.L antibody, LOC738738 antibody, arpc3 antibody, LOC100036831 antibody, LOC100341254 antibody

Background: P21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).Synonyms: Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, p21-ARC

Gene ID: 10094

NCBI Accession: NP_005710

UniProt: O15145

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization