PUM2 antibody (Internal Region)

Catalog No. ABIN374533

Product Details anti-PUM2 Antibody

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Binding Specificity: Internal Region

Reactivity: Human, Chimpanzee

Host: Goat

Clonality: Polyclonal

Conjugate: This PUM2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-DQNRDLKQGD DDDSK

Specificity: This antibody recognizes Pumilio-2.

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence from the internal region of the protein sequence Genename: PUM2

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 1 - 3 μg/mL, Some minor bands are resistant to blocking by the immunizingpeptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PUM2

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Alternative Name: Pumilio 2 / PUM2 (PUM2 Products)

Synonyms: pum-2 antibody, 5730503J23Rik antibody, Pumm2 antibody, mKIAA0235 antibody, PUMH2 antibody, PUML2 antibody, pumilio RNA binding family member 2 antibody, pumilio RNA-binding family member 2 antibody, pum2 antibody, Pum2 antibody, PUM2 antibody

Background: Pumilio 2, a human homolog of Pumilio, a protein required to maintain germ line stem cells in Drosophila and Caenorhabditis elegans, forms a stable complex with DAZ through the same functional domain required for RNA binding, protein-protein interactions and rescue of Pumilio mutations in flies. Pumilio 2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells. Pumilio 2 is a component of conserved cellular machinery that may be required for germ cell development.Synonyms: KIAA0235, PUM-2, PUMH2, Pumilio homolog 2, Pumilio-2

Gene ID: 23369

NCBI Accession: NP_056132

UniProt: Q8TB72

Pathways: Ribonucleoprotein Complex Subunit Organization