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BAD antibody (pSer134)

The Rabbit Polyclonal anti-BAD antibody has been validated for WB and IHC (p). It is suitable to detect BAD in samples from Human.
Catalog No. ABIN389519

Quick Overview for BAD antibody (pSer134) (ABIN389519)

Target

See all BAD Antibodies
BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This BAD antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB6945
  • Binding Specificity

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    pSer134

    Predicted Reactivity

    M, Rat

    Purification

    This antibody is purified through a protein A column, followed by peptide affinity purification.

    Immunogen

    This Bad Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human Bad.

    Isotype

    Ig Fraction
  • Application Notes

    WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

    Expiry Date

    6 months
  • Target

    BAD (BCL2-Associated Agonist of Cell Death (BAD))

    Alternative Name

    Bad

    Background

    Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation

    Molecular Weight

    18392

    Gene ID

    572

    NCBI Accession

    NP_004313, NP_116784

    UniProt

    Q92934

    Pathways

    MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events
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