GST antibody (Alkaline Phosphatase (AP))
Quick Overview for GST antibody (Alkaline Phosphatase (AP)) (ABIN401142)
Target
See all GST AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purification
- Immunoaffinity chromatography
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Immunogen
- Glutathione-S-Transferase [Schistosoma japonicum]
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Isotype
- IgG
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Application Notes
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ELISA: 1/1000 - 1/5000. Western Blot: 1/500 - 1/2000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
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Buffer
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- DO NOT FREEZE!!
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Storage
- 4 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- GST (Glutathione S Transferase (GST))
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Alternative Name
- Glutathione-S-Transferase (GST)
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Background
- GST (Glutathione S-Transferase) is a 26 kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.Synonyms: GST26-Tag, Glutathione S-transferase Tag
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Gene ID
- 6182
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UniProt
- P08515
Target
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