HHV8 antibody (C-Term)

Catalog No. ABIN401445

Product Details anti-HHV8 Antibody

Target: HHV8 (Herpes Virus Type 8 (HHV8))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HHV8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/100,000 - 1/170,000. Western Blot: 1/5,000 - 1/8,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.55 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for HHV8

Target: HHV8 (Herpes Virus Type 8 (HHV8))

Alternative Name: Herpes Virus Type 8 / HHV8 (HHV8 Products)

Target Type: Virus

Background: ORF57 (also known as MTA), one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed, is essential for virus lytic replication. A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein, and those examined act post-transcriptionally, affecting RNA splicing and transport. KSHV ORF57 is capable of establishing both lytic and latent replication cycles. In KS, the virus localizes to tumor progenitor endothelial cells, most of which are latently infected. In cell culture, KSHV replication is generally studied using B-cell lines, such as BCBL-1, generated from primary effusion lymphoma material. Most BCBL-1 cells are latently infected, although there is some spontaneous virus reactivation. Addition of chemical inducers such as sodium n-butyrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), and valproic acid (VA) to these cells efficiently induces the lytic cycle and produces virions. KSHV ORF57 protein predominantly localizes to the nucleus and can shuttle between the nucleus and cytoplasm. Most HSV-1 genes are unspliced, by contrast, ORF57 is spliced gene, the protein is 455 amino acids in length and 50 kDa in size.Synonyms: HHV-8, KHSV