GFP antibody (Texas Red (TR))

Catalog No. ABIN401757

The Texas Red (TR)-conjugated Mouse Monoclonal anti-GFP antibody (Clone 9F9-F9) (ABIN401757) specifically detects GFP in WB, IF and EIA. The antibody is reactive with Aequorea victoria samples.

Quick Overview for GFP antibody (Texas Red (TR)) (ABIN401757)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Texas Red (TR)

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: 9F9-F9

Product Details anti-GFP Antibody (Texas Red (TR))

Specificity: This antibody reacts to Green Fluorescent Protein (GFP).

Characteristics: Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 3.0 moles Texas Red(TM) per mole of Mouse IgG

Purification: Affinity chromatography on Protein A

Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western Blot. Immunohistochemistry.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm where ε0.1% = 0.71)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution stable for a short term at 2-8 °C, but best stored at -20 °C. Following reconstitution store the antibody at -20 °C. Do not aliquot in less than 100 μl.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

Gene ID: 6100

UniProt: P42212