This monoclonal antibody reacts with natural and recombinant human GM-CSF. Does not show any cross reaction with other human Cytokines or Growth Factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL1-beta, TGFbeta-1 and TNF-alpha.
CSF2
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: This HRP-conjugated monoclonal antibody can be used as a Tracer/Detectionantibody in Sandwich ELISA applications for human GM-CSF detection in combination witha Capture antibody (Cat#DM1014). Suggested Capture Coating Dose: approx. 0.3 μg/mL. Substrate: TMB. If the above suggested conditions are followed approximately 2 pg/mL ofGM-CSF in serum/ plasma or medium can be detected with an assay range of 0-500 pg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
0.01 M PBS, pH 7.0 ± 0.1 in 50 % Glycerol and 0.01 % Thimerosal as a bacteriostat.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim