Peptide ELISA: IREB2 Antibody Detection limit dilution 1/32000. Western blot: 1-3 μg/mL. Approx 110 kDa band observed in Human Liver lysates (calculatedMW of 100 kDa according to NP_004127.1). An additional band of unknown identity wasalso consistently observed at 60 kDa. This band was successfully blocked by incubationwith the immunizing peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Background
Iron regulatory protein 2 (IREB2 or IRP2) is a central regulator of cellular iron homeostasis due to its regulation of specific mRNAs encoding proteins of iron uptake and storage. In mammalian cells, regulation of the expression of proteins involved in iron metabolism is achieved through interactions of iron-sensing proteins known as iron regulatory proteins (IRPs), with transcripts that contain RNA stem-loop structures referred to as iron responsive elements (IREs). Two distinct but highly homologous proteins, IRP1 and IRP2, bind IREs with high affinity when cells are depleted of iron, inhibiting translation of some transcripts, such as ferritin, or turnover of others, such as the transferrin receptor (TFRC). IRPs sense cytosolic iron levels and modify expression of proteins involved in iron uptake, export and sequestration according to the needs of individual cells. Regulating the amount of iron sequestered by ferritin and taken up by transferrin receptor ensures that cells acquire sufficient iron for their needs, while preventing iron toxicity.Synonyms: IRE-BP2, IRP2, Iron regulatory protein 2, Iron-responsive element-binding protein 2