Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
PBS, 0.09 % azide, 50 % glycerol
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Aliquot to Avoid freeze/thaw cycles.
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid freeze/thaw cycles.