ABCA5 antibody (AA 1296-1345)
-
- Target See all ABCA5 Antibodies
- ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
-
Binding Specificity
- AA 1296-1345
-
Reactivity
- Human, Dog, Rabbit, Monkey
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This ABCA5 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human ABCA5
- Predicted Reactivity
- Percent identity by BLAST analysis: Human (100%) Mouse, Rat (92%) Dog, Rabbit (85%).
- Purification
- Immunoaffinity purified
- Immunogen
-
Synthetic peptide located between aa1296-1345 of human ABCA5 (Q8WWZ7, NP_061142). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Mouse, Rat, Panda (92%), Bat (90%), Elephant, Dog, Rabbit, Horse, Opossum (85%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
-
-
- Application Notes
-
Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Distilled Water.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
-
- Target
- ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))
- Alternative Name
- ABCA5 (ABCA5 Products)
- Synonyms
- zgc:163009 antibody, ABC13 antibody, EST90625 antibody, B930033A02Rik antibody, mKIAA1888 antibody, ATP binding cassette subfamily A member 5 antibody, ATP-binding cassette, sub-family A (ABC1), member 5 antibody, ATP-binding cassette sub-family A member 5 antibody, ABCA5 antibody, abca5 antibody, LOC100545445 antibody, Abca5 antibody
- Background
-
Name/Gene ID: ABCA5
Subfamily: ATP-binding cassette - ABCA/ABC1
Family: Transporter
Synonyms: ABCA5, ATP-binding cassette A5, ABC13, EST90625, KIAA1888 - Gene ID
- 23461
- NCBI Accession
- NP_061142
- UniProt
- Q8WWZ7
-