GRIA4 antibody (AA 279-292)

Catalog No. ABIN462292

Product Details anti-GRIA4 Antibody

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Binding Specificity: AA 279-292

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Cow, Dog, Horse, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GRIA4 / GLUR4. This antibody is expected to recognize all reported isforms (NP_000820.3, NP_001070711.1, NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus (100%) Lizard (93%) Xenopus, Stickleback, Pufferfish, Zebrafish, Trachemys (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KKLDQREYPGSETP from an internal region of human GRIA4 / GLUR4 (NP_000820.3, NP_001070711.1, NP_001070712.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus (100%), Lizard (93%), Xenopus, Stickleback, Pufferfish, Zebrafish, Trachemys (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 90 kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kD according to NP_001070711.1). Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRIA4

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Alternative Name: GRIA4 / GLUR4 (GRIA4 Products)

Synonyms: GLUR4 antibody, GLUR4C antibody, GLURD antibody, GluA4 antibody, GluR4 antibody, GluR4d antibody, GLUR4/D antibody, glur4 antibody, GluR-D antibody, Glur-4 antibody, Glur4 antibody, Gluralpha4 antibody, spkw1 antibody, DKFZp459P0616 antibody, glur4a antibody, gria4 antibody, iGluR4 antibody, ZfGluR4b antibody, fj46g06 antibody, glur4b antibody, wu:fj46g06 antibody, glutamate ionotropic receptor AMPA type subunit 4 antibody, glutamate receptor, ionotropic, AMPA 4 antibody, glutamate receptor, ionotropic, AMPA4 (alpha 4) antibody, glutamate receptor, ionotropic, AMPA 4a antibody, glutamate receptor, ionotropic, AMPA 4b antibody, GRIA4 antibody, gria4 antibody, Gria4 antibody, gria4a antibody, gria4b antibody

Background: Name/Gene ID: GRIA4
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIA4, GluA4, GluR-4, GLUR4, GLUR4C, Glutamate receptor type d, GluR-D, GLURD, Glutamate receptor 4

Gene ID: 2893

NCBI Accession: NP_000820, NP_001070711, NP_001070712

UniProt: P48058

Pathways: PI3K-Akt Signaling