Cytokeratin 13 antibody (AA 444-458)

Catalog No. ABIN462294

Product Details anti-Cytokeratin 13 Antibody

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Binding Specificity: AA 444-458

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human KRT13 / Cytokeratin 13. This antibody is expected to recognize the reported isoform a (NP_002265.2) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TSNASGRRTSDVRRP from the C-terminus of human KRT13 / Cytokeratin 13 (NP_705694.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000, 0.1 - 0.3 μg/mL), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 55kD band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kD according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr,149(Pt 4):961-71., PMID: 12686638). Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: KRT13 / CK13 / Cytokeratin 13 (KRT13 Products)

Synonyms: krt13 antibody, CK13 antibody, K13 antibody, Ka13 antibody, Krt-1.13 antibody, Krt1-13 antibody, ck13 antibody, k13 antibody, keratin 24 antibody, keratin 13 antibody, keratin 13, type I S homeolog antibody, krt24 antibody, KRT13 antibody, Krt13 antibody, krt13.S antibody, k13 antibody

Background: Name/Gene ID: KRT13

Synonyms: KRT13, CK13, Cytokeratin 13, Cytokeratin-13, CK-13, K13, Keratin 13, Keratin-13

Gene ID: 3860

NCBI Accession: NP_705694