APOB antibody
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- Target See all APOB Antibodies
- APOB (Apolipoprotein B (APOB))
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This APOB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Typically less than 1 % cross reactivity againstother types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible
- Purification
- Immunoaffinity purified
- Immunogen
- Apolipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
- Top Product
- Discover our top product APOB Primary Antibody
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- Application Notes
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Approved: ELISA (1:2000 - 1:10000), IHC, IHC-P (5 μg/mL), IP, WB (1:2000 - 1:10000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
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- Target
- APOB (Apolipoprotein B (APOB))
- Alternative Name
- Apolipoprotein B / APOB (APOB Products)
- Synonyms
- FLDB antibody, LDLCQ4 antibody, Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, AI315052 antibody, apob-100 antibody, apob-48 antibody, APOB-100 antibody, ApoB(100) antibody, APOB antibody, apolipoprotein B antibody, APOB antibody, Apob antibody
- Background
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Name/Gene ID: APOB
Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48 - Gene ID
- 338
- UniProt
- P04114
- Pathways
- Lipid Metabolism
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