Cadherin 5 antibody

Catalog No. ABIN462465

Product Details anti-Cadherin 5 Antibody

Target: Cadherin 5 (CDH5)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Purification: Affinity purified

Immunogen: Human CDH5

Clone: 16B1

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (20 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.Tested in Western blot using Human Umbilical Vein Endothelial Cell (HUVEC) lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: CD144 / CDH5 / VE Cadherin (CDH5 Products)

Synonyms: 7B4 antibody, AA408225 antibody, Cd144 antibody, VE-Cad antibody, VECD antibody, VEcad antibody, Vec antibody, wu:fc88a07 antibody, CD144 antibody, cadherin-5 antibody, cadherin 5 antibody, CDH5 antibody, Cdh5 antibody, cdh5 antibody

Background: Name/Gene ID: CDH5
Family: Cadherin

Synonyms: CDH5, 7B4, 7B4 antigen, CD144, Cadherin-5, Endothelial-specific cadherin, Vascular endothelial cadherin, VE-cadherin, VEC, CD144 antigen, VE Cadherin

Gene ID: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2